How to Read an HPLC Chromatogram for Peptides
An HPLC chromatogram plots retention time vs detector response. The main peak area percent gives purity. Learn to read peptide COA chromatograms.
Tag: research grade peptide
HPLC vs Mass Spectrometry in Peptide Testing
HPLC measures peptide purity and mass spectrometry confirms molecular identity. Both tests are required for a valid research peptide COA.
Lot-Specific vs Generic COA: What to Verify
A lot-specific COA links a unique batch number to the HPLC and mass spec test data for that exact vial, enabling full batch traceability
Acetate vs TFA Salt Form in Peptide Research
Acetate and TFA are the two most common counterions in research peptides. TFA adds more non-active mass and may lower pH in sensitive cell assays.
TFA in Peptides: Residual Salt and Purity Research
TFA (trifluoroacetic acid) is a standard counterion from HPLC peptide purification. It adds 5 to 15 percent non-active mass to labeled vial weight.
Net Peptide Content vs Gross Weight Explained
Net peptide content is the true mass of active peptide in a vial. Gross weight adds counterion and water that can overstate content by 10 to 30 percent.
Why Peptides Are Swirled, Not Shaken: Aggregation
Peptide vials must be swirled, not shaken. Shaking creates shear forces that trigger aggregation and reduce sample purity in research assays.
How Is BPC-157 Reconstituted? A Research Guide
BPC-157 reconstitution is the lab process of dissolving freeze-dried peptide in bacteriostatic water for sample preparation in research assays.
Why Peptide Vials Are Vacuum-Sealed: Stability Data
A peptide vial vacuum seal removes oxygen and moisture before hermetic closure, protecting freeze-dried peptides from oxidation and hydrolysis.
Cloudy Peptide Vial: What Research Says About It
A cloudy peptide vial signals aggregation, precipitation, or bacterial growth. Learn how each cause differs and how to prevent it.