AICAR (5-aminoimidazole-4-carboxamide ribonucleotide)

Description

AICAR — AMPK-Activating Nucleotide Analogue

AICAR (5-aminoimidazole-4-carboxamide ribonucleotide) is a cell-permeable nucleotide analogue of adenosine monophosphate, engineered to activate AMP-activated protein kinase (AMPK). It is supplied for in vitro laboratory research use only.

Research context

Published biochemical literature has examined AICAR’s intracellular conversion to ZMP and its downstream allosteric activation of AMPK, characterised using kinase-activity and phosphorylation-state assays in cultured cell-line systems. In vitro studies in isolated myotube and hepatocyte cell cultures have examined AMPK-pathway effects on glucose-transporter trafficking and fatty-acid oxidation enzyme activity at the cellular level. Kinase-activity assays and cell-based AMPK-pathway reporter systems remain the primary in vitro methodologies applied to this compound. Metabolic-flux assays using radiolabelled glucose and fatty-acid substrates have additionally been used to quantify pathway activity in these cell-culture systems following AMPK activation, with parallel immunoblot analysis of downstream phosphorylation targets across multiple independent cell-line passages and several incubation time points.

Specification

• Available sizes: 50mg · 100mg
• Form: lyophilised powder
• Purity: ≥99% (HPLC)
• Testing: independently lot-tested by Horizon Analytical · Certificate of Analysis per lot

For laboratory research use only. This material is supplied to qualified researchers and institutions for in vitro investigational use. It is not a drug, food, cosmetic or medical device. It is not intended for human or veterinary use, for diagnosis, treatment, cure or prevention of any disease, or for any use outside a controlled laboratory environment. No statement on this site has been evaluated by the Food and Drug Administration. Keep out of reach of children.

Related Research

Compound family: 5-AMINO-1MQ, SLU-PP-322, MOTS-c, NAD+

Browse: Metabolic Research · All Research Peptides

Reconstitution & Lab Testing: All products independently tested by Horizon Analytical. See our Reconstitution & Lab Testing FAQ. Reconstitution typically requires BAC Water.